Glutamate, one of the two acidic proteinogenic amino acids, is also a key molecule in cellular metabolism. In humans, glutamate plays an important role both in amino acid degradation and disposal of excess or waste nitrogen. Glutamate is the most abundant swift excitatory neurotransmitter in the mammalian nervous system. It is believed to be involved in learning and memory and has appeared to be involved in diseases like amyotrophic lateral sclerosis, lathyrism, autism, some forms of mental retardation and Alzheimer's disease. Glutamic acid is also present in a wide variety of foods, and has been used as a flavor enhancer in food industry. The Glutamate Assay Kit provides a sensitive detection method of the glutamate in a variety of samples. The glutamate Enzyme Mix recognizes glutamate as a specific substrate leading to proportional color development. The amount of glutamate can therefore be easily quantified by colorimetric (spectrophotometry at lambda = 450nm) method.Kit Components:*G3990-10A: Assay Buffer, 1x25mlG3990-10B: Enzyme Mix, 1x1vialG3990-10C: Developer, 1x1vialG3990-10D: Standard (0.1M), 1x0.1mlStorage and Stability:Store the kit at -20°C, protect from light. Allow *Assay Buffer to warm to RT before use. Stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.