Fructose-6-phosphate (F6P) is an important intermediate in the glycolytic pathway which leads from glucose to pyruvate. It is formed from glucose-6-phosphate and is further phosphorylated to fructose-1,6-diphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. The transformation of glucose-6-phosphate is controlled by phosphoglucose isomerase, a very interesting enzyme in that it possesses multiple functions, as an isomerase, a neuroleukin, an autocrine motility factor and a differentiation and maturation mediator.Sample Type:Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth media and food products.Intended Use:USBio's Fructose-6-phosphate BioAssay Kit is a fluorescence-based simple, highly sensitive and rapid means of quantifying F6P in a variety of samples.Sensitivity:~1uM of F6PRange:0.01-0.5nmolesSpecificity:MammalianTest Principle:In the assay, F6P is converted to glucose-6-phosphate which is subsequently oxidized with the generation of a fluorescent product.Kit Components:F6P Assay Buffer: 1x25mlF6P Probe: 1x400ulF6P Enzyme Mix (lyophilized) 1 vialF6P Converter (lyophilized) 1 vialF6P Developer (lyophilized) 1 vialF6P Standard (10umol, lyophilized)Storage and Stability:Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.