Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. USBio's Caspase-8 Apoptosis Detection Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence IETD. The assay is based on detection of cleavage of substrate IETD-AFC (7-amino-4-trifluoromethyl coumarin). IETD-AFC emits blue light.Upon proteolytic cleavage of the substrate by caspases, free AFC emits a yellow-green fluorescence. The increase in the yellow-green fluorescence is proportional to the increase in caspase activity.Kit Contents:Assays25100200400Cell Lysis Buffer25ml100ml100ml100ml2X Reaction Buffer2ml4x2ml16ml32mlIETD-AFC (1mM)0.125ml0.5ml2x.05ml2x1mlDT (1M)0.1ml0.4ml0.4ml0.4mlStorage and Stability: Store kit at -20°C before use. After opening, store Cell Lysis Buffer and 2X Reaction Buffer at 4°C. All reagents are stable for 6 months under proper storage conditions.