The Total RNA Isolation Kit provides a fast, simple, and cost-effective method for the isolation of total RNAfrom the whole blood, mammalian cells and bacterial cells. Detergents and chaotropic salt are used to lysecells and inactivate RNase. The specialized high-salt buffering system allows RNA species bases to bind tothe glass fiber matrix of the spin column while contaminants pass through the column. Impurities areefficiently washed away, and the pure RNA is eluted with the REL Buffer without phenol extraction or alcoholprecipitation. The RNA purified with the Total RNA Isolation Kit is suitable for a variety of routine applications,including the RT-PCR, cDNA Synthesis, Northern Blotting, Differential display, Primer Extension and mRNAselection. The entire procedure can be completed within 25-40 minutes.